Switch off the pump by pressing the button pump on the lc10at 3. Aminex hpx 87c for separating dp 1dp 4 saccharides, is a satisfactory example. Biorad aminex hpx 87h column aminex hpx 87h column, supplied by biorad, used in various techniques. Aminex carbohydrate analysis columns life science research. Jun 25, 1993 both columns have a similar retention behaviour for sugars. This column is optimized for analyzing monosaccharides and also provides class separation of di, tri, and tetrasaccharides. The column was main tained at 85 cc, and the sugars were eluted with milliq millipore corp.
Open the vale of the pump by turning it counter clockwise for 90 degrees 5. Use the aminex hpx 87c hplc column for most general sweetener analyses. Aminex hpx87c column aminex hplc columns are packed with a polymerbased matrix polystyrene divinylbenzene. Cleaning and regeneration of the column aminex hpx87h column cleaning 1 4 hours 1. Aminex hpx 87c column this calciumform column is the column of choice for most general sweetener analyses.
View our extensive range of products and shop online securely. I was using an aminex hpx 87h column in an agilent hplc to detect tricarboxylic acids from krebs cycle. General monosaccharide analysis aminex hpx 87c hplc column for sugar analysis 250 x 4. Optimized usp method for atorvastatin calcium using. They are highly effective for monitoring sugars and organic acids simultaneously, which is a time saving procedure. Compatible replacement for the biorad aminex hpx 87c.
Fumarate and pyruvate were detected and quantified by hplc on an aminex hpx 87h column. Analysis of organic acids in combination with carbohydrates. Sls scientific laboratory supplies ltd lab supplies. As an excellent alternative, rezex rpm provides stronger. Reproducible separation of carbohydrate, oligosaccharide. What is the waters equivalent to the aminex hpx87h column.
However, columns like the aminex hpx87h operate in a mixture of size and. I was using an aminex hpx87h column in an agilent hplc to detect. Agave fructans fractionation the purified extract was fractionated by ultrafiltration with a tangential flow filtration system cogent m1, merck millipore, darmstadt, alemania with 1 and 3 kda mwco cassettes 0. Aminex hpx 87c hplc column for sugar analysis 250 x 4. Biorad aminex hpx87c column use the aminex hpx 87c hplc column for sugar analysis according to usp procedures. Separation of sugar alcohols on the aminex hpx87c column containing added corn syrup. Hplc conditions included an aminex hpx 87c column biorad, hercules, ca, usa, deionized water at 85 c as the mobile phase, flow 0. Using the aminex hpx 87h or the inores 259h column, the analysis must be done at room temperature because sucrose inverts quickly to glucose and fructose under acidic solutions at higher temperatures. Calcium form 9m ligand exchange resin with 8% crosslinking. Quantitation of glucose and fructose in high fructose corn syrup. This shorter column is optimized for analysis of acids, and provides much faster elution times than the longer aminex hpx87h column.
Thus, appropriate selection of counterion can moderate the primary partition mechanism. Aminex hpx 87c column, 250 x 4 mm sugar alcohol standards 30% acetonitrileh 2 o 0. Aminex hpx 87c column aminex hplc columns are packed with a polymerbased matrix polystyrene divinylbenzene. Use the aminex hpx 87n hplc column for the analysis of carbohydrates in samples with high salt concentrations. Carefully transfer the line from the eluent bottle to the cleaning solvent 1 4.
Use the aminex hpx 87c hplc column for sugar analysis according to usp procedures. Im looking for an aminex hpx87h equivalent column in. L onto an aminex hpx 87h column biorad as described under materials and methods section. Compare columns from biorad from leading suppliers on biocompare. It is used primarily for the quantitation of glucose and fructose in high fructose corn syrup, and for general monosaccharide analysis. Acids chrompack carbohydrates ca chrompack carbohydrates pb mn nucleogel sugar ca nucleogel sugar pb supelco supelcogel h supelcogel ca supelcogel pb transgenomic icsep cn2 carbosep cho620 carbosep cho682 lead carbosep cho820 carbosep coregel87p. Production, purification and properties of xylanase from a. Guidelines for use and care of aminex resinbased columns. It is optimized for analyzing monosaccharides and also provides class separation of di, tri, and tetrasaccharides. The important difference between this methods is the column temperature. Guidelines for use and care of aminex resin based columns. See the aminex hplc columns selection guide for a complete range of aminex columns for various applications.
Im looking for an aminex hpx87h equivalent column in uplc. Ionexchange highperformance liquid chromatography in the. They separate compounds using the ionmoderated partition chromatography technique. Biorad aminex hpx 87c for separating dp 1dp 4 saccharides and aminex hpx 42c or hpx 42a for separating dp 1dp 7 saccharides. The aminex hpx 87c column resolves the alpha and beta anomers of glucose, but the aminex hpx 87h column, while it provides similar retention, does not resolve the anomers. The aminex hpx 87c column is the column of choice for most general sweetener analyses. Do not operate hightemperature aminex carbohydrate columns hpx 87c, hpx 87p, hpx 87n, or hpx 87k columns above 0.
Sls are the uks largest independent supplier of chemicals, laboratory equipment and consumables. Do not operate high temperature aminex carbohydrate columns aminex hpx 87c, aminex hpx 87p, aminex hpx 87n, or aminex hpx 87k columns above 0. Biorad aminex hpx87c column for sale labx ad 12090529. Aminex hpx 87c app id 14975 app id 14976 03 phenomenex rezex vs. Fast your inquiry will be delivered straight to the manufacturer.
Optimized usp method for atorvastatin calcium using kinetex. A biorad aminex hpx87c is commonly recommended, but the direct replacement rezex rcm provides sharper peak shape and higher resolution, as shown by the comparison below. A simple isocratic elution with slightly acidified water is all that it takes to analyze organic acids in most samples, and filtration is the only sample preparation required before injection. Increase the flow rate of a heated column only after the column has come up to operating temperature. Relation between retention k and carbon number n for. It is used primarily for the quantitation of glucose and fructose in high fructose. Aminex carbohydrate analysis columns separate compounds using a combination of size exclusion and ligand exchange mechanisms. The filtered sample was then injected into an aminex hpx 87p column 300. Biorad aminex hpx 42c aminex hpx 87h aminex hpx 87c aminex hpx 87p varian chrompack org. Id leadform column is optimized for 5 minute analysis of sucrose, glucose, galactose, and fructose. Excess water was then removed from the biomass by manually. Two hundred microliters of each sample was injected on to an aminex hpx 87h column 300 by 7. Hpx87c, aminex hpx87h, and other aminex hpx87 series columns is. Reproducible separation of carbohydrate, oligosaccharide, and.
These columns are macroreticular polystyrene sulfonate divinylbenzene, 28% crosslinked, 825 m particle size. Organic acids and other degradation products were analyzed on a biorad aminex hpx 87h chromatography column with a cationh biorad microguard column at 50 c, with a mobile phase of 5 mm sulfuric acid at a flow rate of 0. Ionexclusion chromatography determination of organic acid in. Search results for biorad aminex hpx 87h at sigmaaldrich. Protect the analytical column described above, by inserting a deionizing precolumn available from chromatographic column manufacturers. If you have any doubts, read the instructions for the column or ask a. Aminex hpx 42c column x aminex hpx 42a column x 300x7. Aminex hpx87c column this calciumform column is the column of choice for most general sweetener analyses. Mannitol aminex hpx 87c rezex rcm aminex hpx 87h rezex roa hpx 87p rezex rpm columns. Aminex hpx 87h columns are very easy to use, clean up and regenerate. Rather than killing a good rp column, i would purchase a dedicated column for organic acids or cva by having at least 5% methanol in all my mobile phases for the aminex column. Aminex hplc columns for organic acid and alcohol analysis. Peaks were detected by refractive index and identified and quantified by comparison to reten tion times of authentic standards xylose, x2x6.
Can we use amino column instead of aminex column for the. Gc capillary column kromasil c18 akzo neobel column. Carbohydrate, oligosaccharide, and organic acid separations. Aminex hpx 87c hplc column for sugar analysis 300 x 7. Yield calculation the glucose yield in the enzymatic hydrolysis experiments was calculated, based on the. Now i gonna use uplc systems lct premier and synapt to detect it and im looking for an. Can i use an aminex hpx87h column to analyze sugars in. Use the aminex hpx 87p hplc column for the analysis of samples containing different types of sugars. Sugar composition of the soluble fraction of 50 mgml cmh. Designed for the analysis of sugars and sugar alcohols.
1071 202 1046 732 1250 444 367 1810 1157 1432 313 320 478 1687 899 490 335 1668 209 106 617 378